Toxicity of Atmospheric Aerosols: Methodologies & Assays
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Efforts have also been spent to study the mechanisms of cellular signaling, stress, response, and physiological change, as well as complex multi-tissue effects upon PM exposure. These assays are diverse, spanning from in vitro acellular assays (i.e., chemical assays) to in vitro cellular and in vivo assays.
Given that various methodologies and assays have been developed or employed, this digital primer aims to introduce the methodologies and fundamental principles used to study atmospheric aerosols’ toxicity and emerging new techniques. A comprehensive literature review of the scientific findings using these methodologies and assays is not the focus of this primer.
The target audiences are senior undergraduates or beginning graduate students unfamiliar with aerosol science and toxicity measurements. An introductory overview of atmospheric aerosols and the motivations to study their toxicity before a detailed discussion of methodologies and assays in the toxicity research of atmospheric aerosols is provided. Beyond this, Toxicity of Atmospheric Aerosols: Methodologies & Assays serves as an assembly of the various methodologies and assays used in the toxicity research of atmospheric aerosols, saving readers time compared to searching from scattered resources.
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Fobang Liu is an associate professor in the Department of Environmental Science and Engineering at the Xi’an Jiaotong University. He earned his doctoral degree at the Max Planck Institute for Chemistry and then did his postdoctoral training at the Georgia Institute of Technology. His research focuses on understanding the health effects of atmospheric fine particulate matter (PM). In particular, he aims to combine laboratory experiments and field measurements to study the chemistry of PM and develop advanced in vitro and in vivo systems to link PM composition to its toxicity and explore the PM pathological mechanisms.
Nga Lee Ng is the Love Family Professor in the School of Chemical & Biomolecular Engineering and the School of Earth & Atmospheric Sciences at the Georgia Institute of Technology. She earned her doctorate in Chemical Engineering from the California Institute of Technology and was a postdoctoral scientist at Aerodyne Research Inc. Dr. Ng’s research focuses on the understanding of the chemical mechanisms of aerosol formation and composition, as well as their health effects. Her group combines laboratory chamber studies and ambient field measurements to study aerosols using advanced mass spectrometry techniques.
