This manual addresses the design and construction of healthcare facilities supporting the response to Ebola and Marburg (E&M) disease outbreaks, particularly in treatment centres (TCs). It provides technical guidance, minimum requirements, practical tools and layout examples, and references for planning, designing, building, and operating healthcare facilities (TCs and others) to respond to E&M disease outbreaks in low– and middle-income contexts and resource-limited settings. The manual complements other World Health Organization (WHO) literature and contributes to the standardization of facility design and operation for safe and humane care and treatment of patients.